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KMID : 0617319950050010129
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Journal of Pharmacetical Sceiences Ewha Womans University
1995 Volume.5 No. 1 p.129 ~ p.133
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Simple and sensitive laser-induced fluorescence detection for capillary electrophoresis and its application to protein separation
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Lim Heung-Bin
Lee Jong-Jin
Lee Kong-Joo
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Abstract
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A low-cost and highly sensitive laser-induced fluorescence detector for on-column detection systems was constructed and its applications were demonstrated in protein separation by capillary electrophoresis. The limit of detection (LOD) at a signal-to-noise ratio of 2 for Lissamine 20 was about 1.4 × 10 exp(-21) mole at 10 nL sample injection and the relative standard deviation (RSD) for 1.5 × 10 exp(-11) M of Lissamine 20 solution was about 15%. These values are comparable to or even better than those reported using a similar on-column detection system. This sensitive LIF detection was applied for the study of tryptic digestion of insulin and for urinary protein profiling.
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